Publications

Triple stain panel differentiates HCC, hepatic adenoma, and FNH on biopsy. Reticulin is the architectural anchor; GPC3 and GS add immunohistochemical specificity.

Foundational review of trichrome, reticulin, PAS, iron, and copper stains in liver pathology. The primary bench reference.

Non-neoplastic fatty liver can show reticulin loss mimicking HCC; critical caveat for using reticulin alone. Foundational pitfall paper.

Rare HCCs retain reticulin; reticulin alone should not be the sole criterion; glypican-3 and Ki-67 required in equivocal cases.

Reference panel for diagnostic liver pathology: trichrome, reticulin, PAS, PAS-D, iron, rhodanine/orcein; use cases and interpretation.

Head-to-head debate on whether portal inflammation belongs in MASLD activity scoring; NASH CRN system currently does not include portal component.

HSCs, Kupffer cells, LSECs, and cholangiocytes direct hepatocyte metabolism in MASLD; hepatocyte pathology cannot be read in isolation from its stromal context.

Extends the standard 0-2 ballooning score with subcategories; improves interobserver agreement among hepatopathologists; validates clinical significance for NASH staging.

Comprehensive review: regeneration is orchestrated via inflammatory priming, complement activation, mechanosensory signaling, metabolic reprogramming. Not a simple proliferative response.

Reviews the role of polyploidization in the regenerative response; polyploid hepatocytes re-enter mitosis and contribute progeny during regeneration.

Includes detailed morphologic criteria for qualifying hepatocytes in organ-on-chip models; source of quantitative ultrastructural benchmarks.

Includes phase-contrast and fluorescence images of hepatocyte morphology in 2D vs 3D culture; useful for comparing in vitro and in vivo appearances.

Step-by-step collagenase perfusion protocol for murine primary hepatocyte isolation; covers viability assessment, plating density, and common failure modes.

Review proposing that hepatic clearance for UGTs and carboxylesterases, whose active sites face the ER lumen, is limited by transport across intracellular membranes, not just enzyme activity. A framework, not yet tested.

Review of liver organoid systems that add non-hepatocyte cell types, maturation and architecture, and what they still lack as models of homeostasis, metabolic disease and cancer.

Review proposing hepatocyte plasticity as an integrative framework linking zonation, regeneration, lineage conversion, and oncogenesis; covers the molecular switches that allow hepatocytes to shift between fates under injury and disease. Review.

Quantified 364 kinases including 23 drug-metabolizing kinases in human liver S9 (n=50), kidney, lung, intestine, and primary hepatocytes (n=8); four kinases detected across all types; tissue-to-plasma ratios show most plasma kinases derive from shedding. Human; multi-tissue proteomics.

Review comparing primary hepatocytes, tumor-derived lines (HepG2 etc.), and iPSC-derived spheroids/organoids as in vitro models; emphasizes membrane transporter expression as the key gap, with iPSC organoids as the emerging gold standard. Review.

RDLMN assay with weak genotoxin 2-AAF shows comparable micronucleus induction at 6 and 8 weeks of age; PCNA and Ki-67 validated as hepatocyte proliferation indicators; supports OECD integration of the liver micronucleus protocol. Rat; single study.

Review of special histochemical stains used in liver biopsy interpretation: reticulin (architecture), trichrome (fibrosis), copper (Wilson), PAS-diastase (glycogen/alpha-1-antitrypsin), iron (hemosiderosis); practical guide for hepatocyte pathology assessment.